Composite

Part:BBa_K2557030:Design

Designed by: Wang Chunfeng   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-Ubc promoter- EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 687
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 693
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to facilitate the construction of TetO-Ubc promoter -recombinase parts, We added the TetO sequence before the Ubc promoter. However, in the absence of TetR, The TetO sequence does not affect the strength of the promoter, that is, it does not affect the expression of EGFP.


Source

The Ubc promoterand and EFGP were cloned from a plasmid borrowed from other laboratories in the school.

References